<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Guimarães, D.</style></author><author><style face="normal" font="default" size="100%">Carvalho, M. L.</style></author><author><style face="normal" font="default" size="100%">Geraldes, V.</style></author><author><style face="normal" font="default" size="100%">Rocha, I.</style></author><author><style face="normal" font="default" size="100%">Santos, J. P.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Study of lead accumulation in bones of Wistar rats by X-ray fluorescence analysis: aging effect</style></title><secondary-title><style face="normal" font="default" size="100%">Metallomics</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1039/c1mt00149c</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">The Royal Society of Chemistry</style></publisher><volume><style face="normal" font="default" size="100%">4</style></volume><pages><style face="normal" font="default" size="100%">66-71</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The accumulation of lead in several bones of Wistar rats with time was determined and compared
Q3 for the different types of bones. Two groups were studied: a control group (n = 20), not exposed
to lead and a contaminated group (n = 30), exposed to lead from birth, first indirectly through
mother’s milk, and then directly through a diet containing lead acetate in drinking water (0.2%).
Rats age ranged from 1 to 11 months, with approximately 1 month intervals and each of the
collections had 3 contaminated rats and 2 control rats. Iliac, femur, tibia–fibula and skull have
been analysed by energy dispersive X-ray fluorescence technique (EDXRF). Samples of
formaldehyde used to preserve the bone tissues were also analysed by Electrothermal Atomic
Absorption (ETAAS), showing that there was no significant loss of lead from the tissue to the
preservative. The bones mean lead concentration of exposed rats range from 100 to 300 mg g 1
while control rats never exceeded 10 mg g 1. Mean bone lead concentrations were compared and
the concentrations were higher in iliac, femur and tibia–fibula and after that skull. However, of
all the concentrations in the different collections, only those in the skull were statistically
Q4 significantly different (p o 0.05) from the other types of bones. Analysis of a radar chart also
allowed us to say that these differences tend to diminish with age. The Spearman correlation test
applied to mean lead concentrations showed strong and very strong positive correlations between
all different types of bones. This test also showed that mean lead concentrations in bones are
negatively correlated with the age of the animals. This correlation is strong in iliac and femur and
very strong in tibia–fibula and skull. It was also shown that the decrease of lead accumulation
with age is made by three plateaus of accumulation,</style></abstract><notes><style face="normal" font="default" size="100%">JPS-Ref51</style></notes><custom3><style face="normal" font="default" size="100%">papers2://publication/uuid/0D26C694-FCE2-47D1-B63D-1D17AA42B179</style></custom3><label><style face="normal" font="default" size="100%">r05534</style></label></record></records></xml>