@article {110,
	title = {Study of lead accumulation in bones of Wistar rats by X-ray fluorescence analysis: aging effect},
	journal = {Metallomics},
	volume = {4},
	year = {2012},
	pages = {66-71},
	publisher = {The Royal Society of Chemistry},
	abstract = {The accumulation of lead in several bones of Wistar rats with time was determined and comparedQ3 for the different types of bones. Two groups were studied: a control group (n = 20), not exposedto lead and a contaminated group (n = 30), exposed to lead from birth, first indirectly throughmother{\textquoteright}s milk, and then directly through a diet containing lead acetate in drinking water (0.2\%).Rats age ranged from 1 to 11 months, with approximately 1 month intervals and each of thecollections had 3 contaminated rats and 2 control rats. Iliac, femur, tibia{\textendash}fibula and skull havebeen analysed by energy dispersive X-ray fluorescence technique (EDXRF). Samples offormaldehyde used to preserve the bone tissues were also analysed by Electrothermal AtomicAbsorption (ETAAS), showing that there was no significant loss of lead from the tissue to thepreservative. The bones mean lead concentration of exposed rats range from 100 to 300 mg g 1while control rats never exceeded 10 mg g 1. Mean bone lead concentrations were compared andthe concentrations were higher in iliac, femur and tibia{\textendash}fibula and after that skull. However, ofall the concentrations in the different collections, only those in the skull were statisticallyQ4 significantly different (p o 0.05) from the other types of bones. Analysis of a radar chart alsoallowed us to say that these differences tend to diminish with age. The Spearman correlation testapplied to mean lead concentrations showed strong and very strong positive correlations betweenall different types of bones. This test also showed that mean lead concentrations in bones arenegatively correlated with the age of the animals. This correlation is strong in iliac and femur andvery strong in tibia{\textendash}fibula and skull. It was also shown that the decrease of lead accumulationwith age is made by three plateaus of accumulation,},
	isbn = {1756-5901},
	url = {http://dx.doi.org/10.1039/c1mt00149c},
	author = {Guimar{\~a}es, D. and Carvalho, M. L. and Geraldes, V. and Rocha, I. and Santos, J. P.}
}
